Anti-ß2 microglobulin (human, ß2M)
Mouse monoclonal antibody
Cat.No. HYB 290-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
ß2 microglobulin (ß2M) is a 12 kDa nonpolymorphic Ig like protein. It is a non-membrane-anchored glycoprotein and is noncovalently associated with 39-44 kDa polymorphic heavy chains of MHC class I molecules to form HLA class I antigen complex. In association with HLA class I, ß2M is expressed on all leukocytes, platelets, endothelial cells, and epithelial cells.
ß2M isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 290-03 binds to human ß2M
Not determined
Suitable for immunoaffinity purification of ß2M (1). A strong reaction is seen with HYB 290-03 when tested in sandwich ELISA in combination with a polyclonal antibody against ß2M.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/30,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on sandwich ELISA in combination with a polyclonal antibody against the antigen. Users should determine the optimal dilutions for their own purpose.
1. Sen JW, Bergen HR, III, Heegaard NH (2003) On-line immunoaffinity-liquid chromatography-mass spectrometry for identification of amyloid disease markers in biological fluids. Anal Chem 75:1196-1202.
