Anti-Serum albumin (human, denatured, HSA)
Mouse monoclonal antibody
Cat.No. HYB 189-01
Preparation: Supernatant
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Human serum albumin (HSA) consists of a single polypeptide chain of 584 aminoacids, stabilized by 17 disulfide bridges. HSA is the most common protein in serum, it is produced in the liver and the concentration in serum is 35-50 mg/ml. HSA is often used as a carrier molecule because of its binding capacity, and thus mainly functions as the regulator of the colloidal osmotic pressure of the blood. The molecular weight is 67.5 kDa (1).
Denaturated HSA isolated from human plasma
HYB 189-01 is specific for denatured HSA
Epitope specificity differs from HYB 189-09 as determined by inhibition ELISA
HYB 189-01 reacts strongly with denatured HSA. Strong reaction is seen in ELISA with denatured HSA directly coated onto the microtiterwell. In Western blot after SDS-PAGE, HYB 189-01 reacts with denatured HSA in non-reduced form only.
There is no reaction to heat denatured HSA.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
