Anti-Hemopexin
Mouse monoclonal antibody
Cat.No. ABS 013-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Hemopexin is a serum glycoprotein that binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation. Structurally hemopexin consists of two similar halves of approximately 200 amino acid residues connected by a histidine-rich hinge region. Each half is itself formed by the repetition of a basic unit of some 35 to 45 residues.
Hemopexin purified from human plasma adsorbed onto aluminum hydroxide gel
ABS 013-04 is specific for human hemopexin
Epitope of ABS 013-04 differs from ABS 013-32
ABS 013-04 binds hemopexin when coated on ELISA wells.
ABS 013-04 (biotinylated) works as a detecton antibody in sandwich ELISA when coating with ABS 013-32.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/10,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 µg/ml. Users should determine the optimal dilutions for their own purposes.
