Anti-Gelsolin (human)
Mouse monoclonal antibody
Cat.No. ABS 017-20
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Gelsolin participates in the extracellular actin scavenger system, binding actin and disaggregating filamentous actin released from dying cells. Actin monomers are then transferred to Gc-globulin. The plasma concentration of gelsolin is reduced in conditions of major actin release, such as acute liver failure, major trauma and rhabdomyolysis, where multi-organ failure is a risk. It has been suggested that gelsolin, like Gc-globulin, can be a prognostic factor in these conditions.
Gelsolin purified from human plasma adsorbed onto aluminum hydroxide gel
ABS 017-20 binds human gelsolin
Not determined
The ELISA dilution guideline applies to gelsolin presented on a coat of polyclonal capture antibody. ABS 017-20 does not work as capture antibody in sandwich ELISA.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Lee PS, Drager LR, Stossel TP, Moore FD, Rogers SO (2006) Relationship of plasma gelsolin levels to outcomes in critically ill surgical patients. Ann Surg 243:399-403.
