BioPorto Diagnostic

AntibodyShop

Gc-globulin (Actin-free) ELISA Kit

Cat.No. KIT 034

Numbers of tests:

96 wells

Assay time:

1 hour

Result type:

Quantitative

Regulatory status:

For in vitro diagnostic use in the European Union only.

For research use only in the rest of the world.

Assess serum Gc-globulin levels to obtain important information about prognosis and progress in critically ill patients suffering from:

Fulminant liver failure
Acetaminophen overdose
Multiple trauma
Sepsis

Using our Gc-globulin (Actin-free) ELISA Kit you can determine Gc-globulin levels in serum or plasma in less than 1 hour.

The fast procedure and easy-to-use ELISA format makes this kit ideal for prognostic assessment and monitoring progress after appropriate treatment has started.

KIT FEATURES

The kit is CE IVD-marked
Results within 1 hour
Highly reproducible and easy to use
- Ready-to-use calibrators and working solutions
- Pre-coated ELISA strips
- All incubations performed at room temperature
Measures Gc-globulin in both serum and plasma
Detection range
- At the recommended sample dilution, the assay range is 5 – 500 µg/L, covering values from normal to critically ill patients
Easy storage
- All components are stored at 4°C
Requires only standard ELISA equipment

References:

Background information:

Harper KD, McLeod JF, Kowalski MA, Haddad JG (1987) Vitamin D binding protein sequesters monomeric actin in the circulation of the rat. J Clin Invest 79:1365-1370.
Haddad JG, Harper KD, Guoth M, Pietra GG, Sanger JW (1990) Angiopathic consequences of saturating the plasma scavenger system for actin. Proc Natl Acad Sci USA 87:1381-1385.Haddad JG (1995) Plasma vitamin D-binding protein (Gc-globulin): multiple tasks. J Steroid Biochem Molec Biol 53:579 582.
Yamamoto N (1996) Structural definition of a potent macrophage activating factor derived from vitamin D3-binding protein with adjuvant activity for antibody production. Mol Immunol 33:1157-1164.
White P, Cooke N (2000) The multifunctional properties and characteristics of vitamin D-binding protein. Tren. Endocrinol Metabol 11:320-327.

Hepatology:

Schiødt FV, Bondesen S, Tygstrup N (1995) Serial measurements of serum Gc-globulin in acetaminophen intoxication. Eur J Gastroenterol Hepatol 7:635-640.
Lee WM, Galbraith RM, Watt GH, Hughes RD, McINtire DD, Hoffman BJ, Williams R (1995) Predicting survival in fulminant hepatic failure using serum Gc protein concentrations. Hepatology 21:101-105.
Schiødt F, Bondesen S, Petersen I, Dalhoff K, Ott P, Tygstrup N (1996) Admission levels of serum Gc-globulin: predictive value in fulminant hepatic failure. Hepatology 23:713-718.
Schiødt FV, Ott P, Bondesen S, Tygstrup N (1997) Reduced serum Gc-globulin levels in patients with fulminant hepatic failure: association with multiple organ failure. Crit Care Med 25:1366-1370.
Schiødt FV, Ott P, Tygstrup N, Dahl B, Bondesen S (2001) Temporal profile of total, bound, and free Gc-globulin after acetaminophen overdose. Liver Transpl 7:732-738.
Schiødt FV, Clemmesen JO, Bondesen S, Dahl, B, Ott P (2001) Increased turnover of Gc-globulin in patients with hepatic encephalopathy. Scand J Gastroenterol 36:998-1003.

Trauma:

Dahl B. Schiødt FV, Nielsen M, Kiær T, Williams JG, Ott P (1999) Admission levels of Gc-globulin predicts outcome after multiple trauma. Injury 30:275-281.
Dahl B, Schiødt FV, Ott P, Wians F, Lee WM, Balko J, O Keefe GE (2003) Plasma concentration of Gc-globulin is associated with organ dysfunction and sepsis after injury. Crit Care Med 31:152-156.

Sepsis:

Lee WM, Reines D, Watt GH, Cook JA, Wise WC, Halushka PV, Galbraith RM (1989) Alterations in Gc levels and complexing in septic shock. Circ Shock 28:249-255
Dahl B, Schiødt FV, Ott P, Wians F, Lee WM, Balko J, O Keefe GE (2003) Plasma concentration of Gc-globulin is associated with organ dysfunction and sepsis after injury. Crit Care Med 31:152-156.

vitamin d binding protein