Anti Apolipoprotein B (human)
mouse monoclonal antibody
Cat.No. HYB 069-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Human LDL
HYB 069-04 has specificity for apo B from human serum/plasma. No crossreactivity is seen with human apolipoprotein A-1.
Epitope differs from that of HYB 069-02 and HYB 069-03
HYB 069-04 reacts with apo B from human serum/plasma when tested in ELISA
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
2. Micic S, Arends J, Norgaard-Pedersen B, Christoffersen K, Andersen GE (1988) Simultanous quantification by double rocket immunoelectrophoresis of apoliproteins A-1 and B in blood spotted on filter paper. Clin Chem 34:2452-2455.
