Anti Apolipoprotein B (human)
mouse monoclonal antibody
Cat.No. HYB 069-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent:0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Apolipoprotein B (apo B) in human plasma is a major protein of low density lipoproteins (LDL). The molecular mass is 260 kDa. Apo B binds to specific receptors on cell membranes and is involved in removal of LDL and very low density lipoprotein (VLDL) cholesterol from circulation.
Human LDL
HYB 069-02 has specificity for apo B from human serum/plasma. No crossreactivity is seen with human apolipoprotein A-1.
Epitope differs from that of HYB 069-03 and HYB 069-04
HYB 069-02 reacts with apo B from human serum/plasma, when used in sandwich ELISA in combination with a polyclonal antibody against apo B, and also with isolated LDL directly coated onto the microtiter plate.
In Western blotting after SDS-PAGE HYB 069-02 binds to apo B in reduced as well as in non-reduced forms, using isolated LDL as antigen.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:32,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Micic S, Arends J, Norgaard-Pedersen B, Christoffersen K, Andersen GE (1988) Simultanous quantification by double rocket immunoelectrophoresis of apoliproteins A-1 and B in blood spotted on filter paper. Clin Chem 34:2452-2455.
2. Young SG, Smith RS, Hogle DM, Curtiss LK, Witztum JL (1986) Two new monoclonal antibody-based enzyme-linked assays of apolipoprotein B. Clin Chem 32:1484-1490.
