Anti-Apolipoprotein A-1 (human)
Mouse monoclonal antibody
Cat.No. HYB 069-01
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. The concentration in plasma is 270-380 mg/100 ml (1).
Apo A-1 isolated from human plasma adsorbed onto aluminum hydroxide gel (2)
HYB 069-01 is specific for apo A-1 from human serum/plasma. No cross-reactivity is seen with human apolipoprotein B.
Not determined
HYB 069-01 reacts with apo A-1 from human serum/plasma when tested in sandwich ELISA in combination with a polyclonal antibody against apo A-1 and also in ELISA with purified apo A-1 or HDL directly coated onto the microtiterwell. In Western blot after SDS-PAGE, HYB 69-01 reacts with apo A-1 in reduced as well as in non-reduced forms.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
2. Micic S, Arends J, Norgaard-Pedersen B, Christoffersen K, Andersen GE (1988) Simultanous quantification by double rocket immunoelectrophoresis of apoliproteins A-1 and B in blood spotted on filter paper. Clin Chem 34:2452-2455.
