Anti a1-antitrypsin (human, native and denatured)
Mouse monoclonal antibody
Cat.No. HYB 191-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
The serum glycoprotein a1-antitrypsin also called a1- AT, is a typical member of the serpin family (serine protease inhibitors), and, an inhibitor of trypsin. a1- AT is synthesized in the liver thus as a single polypeptide chain of approximately 51 kDa showing a marked degree of genetic polymorphism due to the presence of over 20 different alleles. Individuals homozygous for a1- AT deficiency are predisposed to pulmonary emphysema and hepatic cirrhosis.
a1- AT isolated from human plasma
HYB 191-01 is specific for human a1- AT (native and denatured).
Epitope specificity differs from that of HYB 185-02.
Reacts strongly with a1-AT, Strong reaction is seen in ELISA with native and denatured a1- AT coated directly onto the microtiter well. Detect only native a1- AT in sandwich ELISA with a polyclonal capture antibody (e.g. DAKO A0012). Reacts with native and denatured (released and unreleased) a1-AT in imunoblotting. In Western blotting after SDS-PAGE, HYB 191-01 reacts with native and denatured a1- AT in reduced as well as non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:15,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
