Anti a1-antitrypsin (human, alpha 1AT)
Mouse monoclonal antibody
Cat.No. HYB 185-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
The serum glycoprotein a1-antitrypsin also called a1- AT, is a typical member of the serpin family (serine protease inhibitors), and, an inhibitor of trypsin. a1- AT is synthesized in the liver thus as a single polypeptide chain of approximately 51 kDa showing a marked degree of genetic polymorphism due to the presence of over 20 different alleles. Individuals homozygous for a1- AT deficiency are predisposed to pulmonary emphysema and hepatic cirrhosis.
a-1 AT isolated from human plasma
HYB 185-02 is specific for native human a-1 AT
Epitope specificity differs from that of HYB 191-01.
Reacts strongly with native a-1 AT. Strong reaction is seen in ELISA with native a-1 AT directly coated onto the microtiter well. In Western blotting after SDS-PAGE, HYB 185-02 reacts with native a-1 AT in reduced as well as non-reduced form.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Yes | 1:8000 | |
| Immunohistochemistry | Not detemined |
