Anti-alpha1-antitrypsin (human, alpha 1-AT)
Mouse monoclonal antibody
Cat.No. HYB 185-02
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
The serum glycoprotein alpha1-antitrypsin also called alpha1-AT, is a typical member of the serpin family (serine protease inhibitors), and, an inhibitor of trypsin. alpha1- AT is synthesized in the liver thus as a single polypeptide chain of approximately 51 kDa showing a marked degree of genetic polymorphism due to the presence of over 20 different alleles. Individuals homozygous for alpha1- AT deficiency are predisposed to pulmonary emphysema and hepatic cirrhosis.
alpha1-AT isolated from human plasma adsorbed onto aluminum hydroxide gel
HYB 185-02 is specific for native human alpha1-AT
Epitope specificity differs from that of HYB 191-01
Reacts strongly with native alpha1-AT. A strong reaction is seen in ELISA with native alpha1-AT directly coated onto the microtiter well. In Western blotting after SDS-PAGE, HYB 185-02 reacts with native alpha1-AT in reduced as well as non-reduced form. In Western blotting a dilution guideline of 1/8000 has proved successful.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not detemined |
