Anti-Urokinase plasminogen activator receptor (human, uPAR)
Mouse monoclonal antibody
Cat.No. MON R-5
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Urokinase plasminogen activator receptor (uPAR), also known as CD87, is a glycolipid anchored cell surface protein consisting of three homologous domains. It binds uPA with high affinity and enhances and localizes uPA catalyzed plasminogen activation, and thereby plays a key role in cancer invasion and in several tissue remodelling processes (1-3). In addition uPAR also binds to vitronectin and may thereby have biological functions independent of its role in uPA mediated proteolysis (4,5). Elevated levels of uPAR are associated with poor prognosis in many types of cancer (2,3,6,7).
Native human uPAR
MON R-5 is specific for human uPAR. No cross-reactivity is observed with murine uPAR, human uPA, human tPA or human PAI-1 when tested by ELISA and immunoblotting.
MON R-5 reacts with an epitope on domain 1 and differs from that of MON R-3 (8)
MON R-5 reacts with domain 1 (8) when tested by immunoprecipitation of proteolytic fragments of recombinant suPAR. MON R-5 is suitable as detection antibody in ELISA (7,9,10).
| Method | Usability | References |
| ELISA | Yes | 7,9,10 |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Not determined |
1. Llinas P, Le Du MH, Gårdsvoll H, Danø K, Ploug M, Gilquin B, Stura EA, Ménez A (2005) Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide. EMBO J 24:1655-1663.
2. Andreasen PA, Kjøller L, Christensen (1997)The urokinase plasminogen activator system in cancer metastasis. A review. Int J Cancer 2: 1-22.
3. Danø K, Behrendt N, Høyer-Hansen G, Johnsen M, Lund LR, Ploug M, Rømer J (2005) Plasminogen activation and cancer. Thromb Haemo 93:676-681.
4.Mazzier R, Blasi F (2005) The urokinase receptor and the regulation of cell proliferation. Thromb Haemo 93:641-646.
5. Madsen CD, Ferraris GM, Andolfo A, Cunningham O, Sidenius NJ (2007) uPAR-induced cell adhesion and migration: vitronectin provides the key. Cell Biol 177:927-939.
6. Stephens RW, Nielsen HJ, Christensen IJ, Thorlacius-Ussing O, Danø K, Brünner N (1999) Plasma urokinase receptor in colorectal cancer patients: Relation to prognosis. J Natl Cancer Inst 91:869-874.
7. Grøndahl-Hansen J, Peters HA., van Putten WLJ, Look MP, Pappot H, Rønne E, Danø K, Klijn JGM, Brünner N, Foekens JA (1995) Prognostic significance of the receptor for urokinase plasminogen activator in breast cancer. Clin Cancer Res 1:1079-1087.
8. Rønne E, Behrendt N, Ellis V, Ploug M, Danø K, Høyer-Hansen G (1991) Cell induced potentiation of the plasminogen activation system is abolished by a monoclonal antibody that recognizes the NH2 -terminal domain of the urokinase receptor. FEBS Lett 288:233 236.
9. Stephens RW, Pedersen AN, Nielsen HJ, Hamers MJAG, Høyer-Hansen G, Rønne E, Dybkjær E. Danø K, Brünner N (1997) ELISA determination of soluble urokinase receptor in blood from healthy donors and cancer patients. Clin Chem 43:1868-1876 .
10. Rønne E, Høyer-Hansen G, Brünner N, Pedersen H, Rank F, Osborne CK, Clark GM, Danø K, Grøndahl-Hansen J (1995) Urokinase-receptor in breast cancer tissue extracts. Enzyme-linked immunosorbent assay with a combination of mono- and polyclonal antibodies. Breast Cancer Res Treatm 33:199-207.
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