Anti Thioguanine (2-amino-6-mercaptopurine)
Mouse monoclonal antibody
Cat.No. HYB 138-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Thioguanine is a purine analogue used in cancer chemotherapy. It is the 6-thione analogue of the endogenous 6-OHpurine base guanine. In the cell thioguanine is converted to the ribonucleotides: 6-thioguanosine-5'-phosphate and 6-thioinosine-5'-phosphate. These fraudulent nucleotides produce their cytotoxic effects by several mechanisms, e.g. inhibitory action on de novo purine synthesis, and may themselves be incorporated into DNA. Thioguanine hasa molecular weight of 167 Da (1,2).
S-substituted thioguanine linked to PPD (2)
HYB 138-02 has specificity for thioguanine. Crossreactivity is seen with 6-(methylthio)purine but not with various other thioguanine analogue (2).
HYB 138-02 reacts strongly with thioguanine coupled to a carrier protein via the sulphur atom in the 6-position, and this interaction can be inhibited with free thioguanine (2).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | ||
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Rang HP, Dale MM, Ritter JM (1995) Chemotherapy. Pharmacology. New York: Churchill Livingstone 696-717.
2. Nerstrom VM, Henriksen U, Nielsen PE, Buchardt O, Schmiegelow K, Koch C (1994) Monoclonal antibodies to thioguanine: influence of coupling position on fine specificity. Bioconj Chem 5:357-363.
