Anti Thioguanine (2-amino-6-mercaptopurine)
Mouse monoclonal antibody
Cat.No. HYB 125-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Thioguanine is a purine analogue used in cancer chemotherapy. It is the 6-thione analogue of the endogenous 6-OHpurine base guanine. In the cell thioguanine is converted to the ribonucleotides: 6-thioguanosine-5'-phosphate and 6-thioinosine-5'-phosphate. These fraudulent nucleotides produce their cytotoxic effects by several mechanisms, e.g. inhibitory action on de novo purine synthesis, and may themselves be incorporated into DNA. Thioguanine has a molecular weight of 167 Da (1,2).
9-substituted thioguanine linked to PPD (2)
HYB 125-03 has specificity for thioguanine. Crossreactivity is seen with a variety of thioguanine analogues, except with 6-(methylthio)purine, 6-(methylthio)purine riboside, 6-thioxanthine and 2-amino-8-phenyl-6-purinethione and (2).
HYB 125-03 reacts strongly with free thioguanine and reactivity is primarily dependent on the structure of the pyrimidine ring (2).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | ||
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Rang HP, Dale MM, Ritter JM (1995) Chemotherapy. Pharmacology. New York: Churchill Livingstone 696-717.2. Nerstrom VM, Henriksen U, Nielsen PE, Buchardt O, Schmiegelow K, Koch C (1994) Monoclonal antibodies to thioguanine: influence of coupling position on fine specificity. Bioconj Chem 5:357-363.
