Anti-Ovalbumin (chicken)
Mouse monoclonal antibody
Cat.No. HYB 099-02
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Ovalbumin is the main protein found in egg white, making up 60-65% of the total protein. Native chicken ovalbuminis is a globular monomer with a molecular mass of 45 kDa (1). It is structurally related to both serpins and lipocalins.
Purified chicken ovalbumin adsorbed onto aluminum hydroxide gel
HYB 099-02 reacts with native chicken ovalbumin (2). Cross-reactivity with ovalbumins from other avian species has not been tested.
Epitope specificity differs from that of HYB 099-01 and HYB 099-09, as indicated by lack of mutual inhibition of antigen binding.
HYB 099-02 reacts with ovalbumin in its native form, but does not recognize denatured ovalbumin. HYB 099-02 can be used for immunoprecipitation of ovalbumin.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/32,000 | |
| Immunoblotting | No | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for native ovalbumin coated at 1 µg/mL. Users should determine the optimal dilutions for their own purposes.
1. Nisbet AD, Saundry RH, Moir AJ, Fothergill LA, Fothergill JE (1981) The complete amino acid sequence of hen ovalbumin. Eur J Biochem 115:335-345.
2. Houen G, Koch C (1997) A non-denaturing enzyme linked immunosorbent assay with protein preadsorbed onto aluminum hydroxide. J Immunol Methods 200:99-105.
