Anti MHC class I alpha chain (chicken)
mouse monoclonal antibody
Cat.No. KSK 001-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
The chicken major histocompatibility complex (MHC) class I is similar to the mammalian MHC class I consisting of a polymorphic alpha chain (45 kDa) non covalently bound to b2-microglobulin (12-14 kDa). MHC class I is expressed on most nucleated cells in chicken, including the red blood cells.
White blood cells from chicken plasma
KSK 001-03 reacts with the alpha chain of chicken MHC class I, both native and denatured.
Reacts with a non-polymorphic epitope on chicken MHC class I alpha chain
KSK 001-03 is suitable for Western blotting, flow cytometry, immunoprecipitation, and affinity purification on chicken MHC class I.
| Method | Usability | Dilution guideline | References |
| ELISA | Not determined | ||
| Immunoblotting | Yes | 3, 4 | |
| Immunohistochemistry | Not determined |
1. Guillemot F, Kaufman JF, Skjoedt K, Auffray C (1989) The major histocompatibility complex in the chicken. Trends Genet 5:300-304.
2. Kaufman J, Skjoedt K, Salomonsen J (1990) The MHC molecules of nonmammalian vertebrates. Immunol Rev 113:83-117.
3. Kaufman J, Skjoedt K, Salomonsen J, Simonsen M, Pasquier L. Du, Parisot R, Riegert P (1990) MHC-like molecules in some nonmammalian vertebrates can be detected by some cross-reactive xenoantisera. J Immunol 144:2258-2272.
4. Dunon D, Kaufman J, Salomonsen J, Skjoedt K, Vainio O, Thiery JP, Imhof BA (1990) T cell precursor migration towards beta 2-microglobulin is involved in thymus colonization of chicken embryos. EMBO J 9:3315-3322.
5. Salomonsen J, Dunon D, Skjodt K, Thorpe D, Vainio O, Kaufman J (1991) Chicken major histocompatibility complex-encoded B-G antigens are found on many cell types that are important for the immune system. Proc.Natl.Acad.Sci.U.S.A 88:1359-1363.
6. Koch C (1986) The alternative complement pathway in chickens. Purification of factor B and production of a monospecific antibody against it. Acta Pathol Microbiol Immunol Scand C 94:253-259.
