Anti Immunoglobulin heavy chain (chicken, IgY)
Mouse monoclonal antibody
Cat.No. HYB 208-03
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl
Storage: In the dark at 4-8ºC
Secreted immunoglobulins (Ig) have two separable functions: one is to bind specifically to pathogenic molecules, the other is to recruit cells and molecules to destroy the opsonized pathogen. IgG in chicken (also called IgY) has a tetrameric structure consisting of two identical light chains (Mr = 24 kDa) and two identical heavy chains (Mr = 73 kDa).
Immunoglobulin isolated from chicken serum
HYB 208-03 is specific for chicken Ig
HYB 208-03 reacts strongly with chicken Ig. Strong reaction is seen in ELISA with chicken Ig directly coated onto the microtiterwell and when tested in sandwich ELISA in combination with a polyclonal antibody against chicken Ig (eg. rabbit anti chicken IgG from Sigma). In Western blotting after SDS-PAGE, HYB 208-03 reacts with chicken Ig heavy chain in both reduced and non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:15,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Leslie GA, Clem LW (1969) Phylogen of immunoglobulin structure and function. 3. Immunoglobulins of the chicken. J Exp Med 130:1337-1352.
2. Warr GW, Magor KE, Higgins DA (1995) IgY: Clues to the origins of modern antibodies. Immunol Today 16:392-398.
