Anti Biotin
mouse monoclonal antibody
Cat.No. HYB 212-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Biotin is a natural occurring vitamin also called vitamin B4. Biotin is required as prosthetic group of enzymes involved in incorporation of carbon dioxide into organic compounds. Biotin has a MW of 244 Da (1).
Biotinylated denatured ovalbumin
HYB 212-01 is specific for biotin
Not determined
HYB 212-01 reacts strongly with biotin. Strong reaction is seen in ELISA with biotin/OA directly coated onto the microtiter well, also when tested in sandwich ELISA in combination with streptavidin. In Western blotting after SDS-PAGE, HYB 212-01 reacts with biotinylated proteins in both reduced and non-reduced forms.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:40,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Scott T & Eagleson M (1988) Concise Encyclopedia Biochemistry: Walter de Gruyter, New York.
