Anti Chloroquine
mouse monoclonal antibody
Cat.No. HYB 317-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Chloroquine is the prototype anti malarial drug, most widely used to treat all types of malarial infections. It is also the cheapest, time tested and safe anti malarial agent. Chloroquine is a hapten of molecular weight 158.29 g/mol.
The quinoline moiety of the chloroquine molecule is the same as for many other antimalarial drugs, but the sidechain is unique.
2-amino-5-diethylaminopentane coupled to immunogenic carrier protein
HYB 317-01 has specificity for chloroquine and can be used for chloroquine quantification in human serum or plasma with a detection limit of app. 20 ng/ml.
HYB 317-01 has specificity for the non-quinoline-sidechain of chloroquine
HYB 317-01 is able to distinguish chloroquine from the metabolite desethylchloroquine and exhibits no crossreactivity to other antimalarial drugs.
| Methode | Usability (yes/no) | Dilution guideline | References |
| ELISA | Yes | ||
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Rowell V, Rowell FJ, Baker A, Laurie D, Sidki AM (1988) A specific ELISA method for determining chloroquine in urine or dried blood spots. Bull World Health Organ 66:211-217.
2. Shenton FC, Bots M, Menon A, Eggelte TA, de Wit M, Greenwood BM (1988) An ELISA test for detecting chloroquine in urine. Trans R Soc Trop Med Hyg 82:216-220.
3. Escande C, Chevalier P, Verdier F, Bourdon R (1990) Sensitive radioimmunoassay and enzyme-linked immunosorbent assay for the simultaneous determination of chloroquine and its metabolites in biological fluids. J Pharm Sci 79:23-27.
