Anti Mycobacterium tuberculosis Ag85
Mouse monoclonal antibody
Cat.No. HYT 27 (WHO no. IT-49)
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
Bacterial press extract from Mycobacterium tuberculosis H37Rv
HYT 27 is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein.
No crossreactivity to antigens from Listeria monocytogenes or Escherichia coli.
Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid).
HYT 27 reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1).
In Western blotting after SDS-PAGE, HYT 27 reacts strongly with Ag85C and weaker with Ag85A and Ag85B.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:400 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1. Schou C, Yuan ZL, Andersen AB, Bennedsen J (1985) Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis. Acta Pathol Microbiol Immunol Scand (C) 93:265-272.
2. Andersen AB, Yuan ZL, Haslov K, Vergmann B, Bennedsen J (1986) Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol 23:446-451.
3. Worsaae A, Ljungqvist L, Heron I (1988) Monoclonal antibodies produced in BALB.B10 mice define new antigenic determinants in culture filtrate preparations of Mycobacterium tuberculosis. J Clin Microbiol 26:2608-2614.
4. Belisle JT, Vissa VD, Sievert T, Takayama K, Brennan PJ, Besra GS (1997) Role of the major antigen of Mycobacterium tuberculosis in cell wall biogenesis. Science 276:1420-22.
5. D'Souza S, Rosseels V, Romano M, Tanghe A, Denis O, Jurion F, Castiglione N, Vanonckelen A, Palfliet K, Huygen K (2003) Mapping of murine Th1 helper T-Cell epitopes of mycolyl transferases Ag85A, Ag85B, and Ag85C from Mycobacterium tuberculosis. Infect Immun 71:483-493.
