Anti-Influenza A virus matrix protein
MMouse monoclonal antibody, biotinylated
Cat.No. HYB 156-02B
Preparation: Biotinylated
Content: 100 µL, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The matrix protein of influenza virus is a multimer of 25-30 kDa subunits that mediates the encapsidation of the RNA-nucleoprotein cores into the membrane envelope (1).
Unpurified influenza A virus (H1N 1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C (2).
HYB 156-02 is specific for influenza A virus matrix protein as determined by Western blotting (2)
Not determined
A pool of HYB 156-01 and HYB 156-02 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using the same pool of antibodies (biotinylated) for detection (2).
HYB 156-02 reacts with influenza A-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/40,000 | 2 |
| Immunoblotting | |||
| Immunohistochemistry |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 1 µg/ml. Users should determine the optimal dilutions for their own purposes.
1. Sha B, Luo M (1997) Structure of a bifunctional membrane-RNA binding protein, influenza virus matrix protein M1. Nat Struct Biol 4:239-244.
2. Glikmann G, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with respiratory infections. Clin Diagn Virol 3:361-369.
