Anti-Influenza A virus nucleoprotein
Mouse monoclonal antibody, biotinylated
Cat.No. HYB 156-01B
Preparation: Biotinylated
Content: 100 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
IInfluenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1).
Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37°C (2).
HYB 156-01 is specific for influenza A virus nucleoprotein and does not cross-react with influenza B virus (2).
Not determined
HYB 156-01 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using HYB 156-01B (biotinylated) for detection (2).
HYB 156-01 reacts with influenza A-infected, acetone-fixed VERO cells in immunofluorescence cytochemistry.
| Method | Usability | References |
| ELISA | Yes | 2 |
| Immunoblotting | Not Determined | |
| Immunoflourescence | Not Determined |
1. Portela A, Digard P (2002) The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol 83:723-734.
2. Glikmann G, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with respiratory infections. Clin Diagn Virol 3:361-369.
