Anti-Influenza A virus nucleoprotein
Mouse monoclonal antibody
Cat.No. HYB 156-01
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Influenza viruses are common and highly infectious human pathogens. They are constantly mutating to modify their antigenicity and avoid elimination by immunity to previous generations of virus. The nucleoprotein of influenza A virus is a basic, phosphorylated multimeric protein consisting of 498 aa subunits that encapsidate the viral RNA genome to form a ribonucleoprotein particle (1)
Unpurified influenza A virus (H1N1 A/PR/8/34) for primary intranasal immunization, boosted intravenously with purified influenza A virus disrupted with Triton X-100 for 45 min at 37ºC (2)
HYB 156-01 is specific for influenza A virus nucleoprotein and does not cross-react with influenza B virus (2)
Not determined
A pool of HYB 156-01 and HYB 156-02 is suitable as capture antibody in a sandwich ELISA for influenza A virus, using the same pool of antibodies (biotinylated) for detection (2).
HYB 156-01 reacts with influenza A-infected, acetone-fixed VERO cells in immunoflourescence cytochemistry
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 2 | |
| Immunoblotting | No | 2 | |
| Immunoflourescence | Yes | 1/1500 |
1. Portela A, Digard P (2002) The influenza virus nucleoprotein: a multifunctional RNA-binding protein pivotal to virus replication. J Gen Virol 83:723-734
2. Glikmann G, Mordhorst CH, Koch C (1995) Monoclonal antibodies for the direct detection of influenza-A virus by ELISA in clinical specimens from patients with respiratory infections. Clin Diagn Virol 3:361-369
Larger Picture (652kB)
HYB 156-01 diluted 1:1500 + secondary polyclonal goat anti-mouse Ig Fluor 555 conjugated antibody (Alexa A21424) 1:200 + Vectashield mounting medium with DAPI. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
Larger Picture (121kB)
HYB 156-01 diluted 1:1000 + secondary polyclonal rabbit anti-mouse Ig FITC conjugated antibody (DAKO F0232) 1:20 + 1% Evans blue colors Influenza A nucleoprotein. DAKO IMAGEN Influenza Virus slides (S6112) were used and the entire infected Vero cells are seen.
Related Articles
Antibody MSDS
April 23 2008
