Anti-Diphtheria toxoid
Mouse monoclonal antibody
Cat.No. HYB 123-09
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Corynebacterium diphtheriae is a Gram-positive, non-motile bacterium that can cause serious illness in unvaccinated humans.C. diphteriae infects the epithelial cells of the upper respiratory tract from where they secrete a potent toxin thar is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. The toxin is a single protein chain of 62 kDa which is "nicked" at proteolytic processing sites to produce an A chain of 21 kDa and a B chain of 40 kDa, held together by a disulfide bridge. The A-chain carries the ADP-ribosylation enzymatic activity responsible for the toxic action (1)
Diphtheria toxoid prepared from toxin purified from C. diphtheriae subsp. intermedius Park-Williams no. 8 by formaldehyde inactivation (2) and adsorbed onto aluminum hydroxide gel.
HYB 123-09 reacts with diphtheria toxoid
Not determined
HYB 123-09 reacts in ELISA wells coated with diphtheria toxoid. When used in Western blotting HYB 123-09 detects a band at approximately 63 kDa corresponding to the toxin proenzyme.
An antibody pair consisting of HYB 123-09 and HYB 123-05 can be used in a sandwich ELISA when using HYB 123-09 as a coat and biotinylated HYB 123-05 a detection antibody
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/8000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 2 µg/mL. Users should determine the optimal dilutions for their own purposes.
1. Pappenheimer AM Jr (1977) Diptheria toxin. Ann Rev Biochem 46:69-94
2. World Health Organization (1977) Manual for the production and control of vaccines. Diphtheria toxoid. BLG/UNDP/77.1 World Health Organization, Geneva
