Anti-Diphtheria toxoid
Mouse monoclonal antibody
Cat.No. HYB 123-05
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Corynebacterium diphtheriae is a Gram-positive, non-motile bacterium that can cause serious illness in unvaccinated humans.C. diphteriae infects the epithelial cells of the upper respiratory tract from where they secrete a potent toxin thar is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body. The toxin is a single protein chain of 62 kDa which is "nicked" at proteolytic processing sites to produce an A chain of 21 kDa and a B chain of 40 kDa, held together by a disulfide bridge. The A-chain carries the ADP-ribosylation enzymatic activity responsible for the toxic action (1)
Diphtheria toxoid prepared from toxin purified from C. diphtheriae subsp. intermedius Park-Williams no. 8 by formaldehyde inactivation (2) and adsorbed onto aluminum hydroxide gel.
HYB 123-05 reacts with diphtheria toxoid (3) and also neutralizes diphtheria toxin as determined in a VERO cell assay.
Not determined
HYB 123-05 reacts strongly with diphtheria toxoid. HYB 123-05 detects diphtheria toxoid in sandwich ELISA in combination with a polyclonal antibody against the toxoid as capture antibody (3). Strong reaction is also seen in ELISA with diphtheria toxoid coated directly onto the microtiter well.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/35000 | |
| immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 2 µg/mL. For use as detection antibody in sandwich ELISA the dilution will typically be lower. Users should determine the optimal dilutions for their own purposes.
1. Pappenheimer AM Jr (1977) Diptheria toxin. Ann Rev Biochem 46:69-94
2. World Health Organization (1977) Manual for the production and control of vaccines. Diphtheria toxoid. BLG/UNDP/77.1 World Health Organization, Geneva
3. Nielsen PB, Koch C, Friis H, Heron I, Prag J, Schmidt J (1987) Double-antibody sandwich enzyme-linked immunosorbent assay for rapid detection of toxin-producing Corynebacterium diphtheriae. J Clin Microbiol 25:1280-1284.
