Anti-Clostridium tetani toxoid (tetanus toxoid)
Mouse monoclonal antibody
Cat.No. HYB 278-15
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Clostridium tetani is a Gram-positive, motile bacterium found in soil and animal feces. Toxigenic strains of C. tetani contain a plasmid encoding the potent exotoxin which causes tetanus (lockjaw). The toxin is synthesized as a single 150 kDa protein chain which is cleaved ("nicked") into a 100 kDa heavy chain (fragment B) and a 50 kDa light chain (fragment A) connected by a disulfide bridge. Tetanus toxoid is produced by prolonged incubation of the toxin with formaldehyde and lysine, and is often used as a carrier for compounds of poor immunogenicity.
Tetanus toxoid, formaldehyde inactivated tetanus toxin
HYB 278-15 binds to both tetanus toxin and tetanus toxoid
Epitope specificity differs from that of HYB 278-14 and HYB 278-17 as determined by competitive ELISA.
HYB 278-15 does not neutralize tetanus toxin efficiently enough to protect mice in the in-vivo test of tetanus antioxin activity.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/16,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 µg/mL. Users should determine the optimal dilutions for their own purposes.
