Anti Clostridium botulinum E toxoid
Mouse monoclonal antibody
Cat.No. HAV 001-06
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Clostridium botulinum is a gram-positive, anaerobic motile bacteria normally found in soil. C. botulinum is a potential threat to both humans and animals through contaminated food because of the potent neurotoxin they produce. C. botulinum bacteria are categorized within 7 strains A-G based on the characteristics of their toxin. A, B, E and F strains are known to cause foodpoisoning in humans.
Clostridium botulinum type E toxoid
HAV 001-06 is specific for botulinum toxoid. No crossreactivity to type A and type F toxoids.
Epitope differs from that of HAV 001-02
HAV 001-02 reacts with botulinum toxoid type E when tested in direct ELISA with type E toxoid coated directly onto the microtiter well. No or very little reaction was seen with toxoids type A and type F.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Prabakaran S, Tepp W, Das Gupta BR (2001) Botulinum neurotoxin types B and E: purification, limited proteolysis by endoproteinase Glu-C and pepsin, and comparison of their identified cleaved sites relative to the three-dimensional structure of type A neurotoxin. Toxicon 39:1515-1531.
