Anti Procollagen type I C-terminal propeptide (human, PICP)
Mouse monoclonal antibody
Cat.No. BTE 002-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Collagen type I is present in soft connective tissue and bone where it represents approximately 90% of the organix matrix. Collagen type I is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. The propeptide levels can be measured in serum as indicators of collagen production. Elevated values of these propeptides in serum are found in patients with various bone metabolism defects. Procollagen type I C-terminal propeptide (PICP) has a molecular weight of approximately 100 kDa and the normal range in serum is 100-300µg/ml.
PICP purified from second trimester amniotic fluid by affinity chromatography
BTE 002-08 is specific for for native PICP from amniotic fluid. Crossreactivity to other species is not determined.
Epitope differs from that of BTE 002-18
BTE 002-08 is especially well suited as catching antibody in a sandwich ELISA in combination with BTE 002-18.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:6000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Hertel NT, Stoltenberg M, Juul A, Main KM, Muller J, Nielsen CT, Lorenzen I, Skakkebaek NE (1993) Serum concentrations of type I and III procollagen propeptides in healthy children and girls with central precocious puberty during treatment with gonadotropin-releasing hormone analog and cyproterone acetate. J Clin Endocrinol Metab 76:924-927.
2. Kjeldsen J, Rasmussen M, Schaffalitzky de Muckadell OB, Kronborg O, Junker P (2001) Collagen metabolites in the peripheral and splanchnic circulation of patients with Crohn disease. Scand J Gastroenterol 36:1193-1197.
3. Orum O, Hansen M, Jensen CH, Sorensen HA, Jensen LB, Horslev-Petersen K, Teisner B (1996) Procollagen type I N-terminal propeptide (PINP) as an indicator of type I collagen metabolism: ELISA development, reference interval, and hypovitaminosis D induced hyperparathyroidism. Bone 19:157-163.
