Anti Pregnancy associated plasma protein A (human, PAPP-A)
Mouse monoclonal antibody
Cat.No. BTE 004-09
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
Purified human PAPP-A
BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
The epitope of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.
In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as detection antibody. BTE 004-09 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.
BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Yes |
1. Folkersen J, Grudzinskas JG, Hindersson P, Teisner B, Westergaard JG (1981) Purification of pregnancy-associated plasma protein-A by a two step affinity chromatographic procedure. Placenta 2:11-17.
2. Sinosich MJ, Teisner B, Folkersen J, Saunders DM, Grudzinskas JG (1982) Radioimmunoassay for pregnancy-associated plasma protein A. Clin Chem 28:50-53.
3. Chemnitz J, Folkersen J, Teisner B, Sinosich MJ, Tornehave D, Westergaard JG, Bolton AE, Grudzinskas JG (1986) Comparison of different antibody preparations against pregnancy-associated plasma protein-A (PAPP-A) for use in localization and immunoassay studies. Br J Obstet Gynaecol 93:916-923.
4. Sinosich MJ, Sim RB, Teisner B (1990) Characterization of pregnancy-associated plasma protein-A: comparison with alpha 2-macroglobulin. Biochem Int 20:579-589.
5. Macintosh MC, Iles R, Teisner B, Sharma K, Chard T, Grudzinskas JG, Ward RH, Muller F (1994) Maternal serum human chorionic gonadotrophin and pregnancy-associated plasma protein A, markers for fetal Down syndrome at 8-14 weeks. Prenat Diagn 14:203-208.
6. Casals E, Fortuny A, Grudzinskas JG, Suzuki Y, Teisner B, Comas C, Sanllehy C, Ojuel J, Borrell A, Soler A, Ballesta AM (1996) First-trimester biochemical screening for Down syndrome with the use of PAPP-A, AFP, and beta-hCG. Prenat Diagn 16:405-410.
Larger Picture (140kB)
Immunohistochemical staining of human placenta tissue using AntibodyShop product BTE 004-09 specific for human pregnancy associated plasma protein A (PAPP-A). Staining on both frozen (red-brown; upper picture) and paraffin embedded (red-brown; lower picture) sections is restricted to the syncytiotrophoblast of the chorionic villi.
Larger Picture (161kB)
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April 23 2008
