Anti Placental protein 14 (human, glycodelin A)
Mouse monoclonal antibody
Cat.No. BTE 001-16
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Human placental protein 14 (PP14; also known as glycodelin and progesterone-associated endometrial protein) is a protein of the lipocalin structural superfamily. PP14 is the most abundant product of the secretory endometrium, and has been proposed as a biochemical marker of endometrial function in women undergoing in vitro fertilization treatment (1).
Placental protein 14 (glycodelin A) purified from second trimester amniotic fluid
BTE 001-16 is specific for human PP14
Differs from that of BTE 001-13 and -18
BTE 001-16 reacts strongly to human PP14 in sandwich ELISA in combination with a polyclonal antibody.
When staining formalin-fixed paraffin-embedded endometrial tissue from the late secretory phase, staining is restricted to the glandular cells.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:200 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Yes |
1. Westergaard LG, Wiberg N, Andersen CY, Laursen SB, Kliem A, Westergaard JG, Teisner B (1998) Circulating concentrations of placenta protein 14 during the natural menstrual cycle in women significantly reflect endometrial receptivity to implantation and pregnancy during successive assisted reproduction cycles. Hum Reprod 13:2612-2619.
2. Hustin J, Philippe E, Teisner B, Grudzinskas JG (1994) Immunohistochemical localization of two endometrial proteins in the early days of human pregnancy. Placenta 15:701-708.
3. Tornehave D, Fay TN, Teisner B, Chemnitz J, Westergaard JG, Grudzinskas JG (1989) Two fetal antigens (FA-1 and FA-2) and endometrial proteins (PP12 and PP14) isolated from amniotic fluid: localisation in the fetus and adult female genital tract. Eur J Obstet Gynecol Reprod Biol 30:221-232.
Larger Picture (402kB)
Immunohistochemical staining of a formalin-fixed, paraffin-embedded biopsy of late secretory phase endometrium, using AntibodyShop product BTE 001-16 specific for PP14 (glycodelin A). Specific staining (red-brown) is restricted to the glandular cells of the endometrium.
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April 23 2008
