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Anti NGAL (rat, neutrophil gelatinase-associated lipocalin)

Mouse monoclonal antibody

Cat.No. ABS 039-08

Subclass IgG1/k

Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 無 and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including cells of the renal proximal tubules. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is upregulated in a variety of pathological situations including infection, inflammation, ischemia-reperfusion and in certain adenocarcinomas.

Immunogen:

Recombinant rat NGAL precursor with C-terminal His-tag coupled to carrier.

Specificity:

ABS 039-08 binds rat NGAL and shows no cross-reaction with human NGAL.

Epitope specificity:

Not determined

Reactivity:

ABS 039-08 binds free rat NGAL in solution. The ELISA dilution guideline applies to rat NGAL coated directly at 1.0 痢/mL.
ABS 039-08 (as capture antibody) forms a sandwich ELISA pair with ABS 039-32 (as biotinylated detection antibody) for measuring rat NGAL, giving detection limit of 0.012 ng/mL in an unoptimized buffer assay.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1:125
Immunoblotting	Not determined
Immunohistochemistry	Not determined

Application Remarks:

References:

1. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-43.

Lipocalin 2