Anti-NGAL (human, neutrophil gelatinase-associated lipocalin)
Mouse monoclonal antibody
Cat.No. HYB 211-05
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B) (1) Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers (2). The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury (3).
Human recombinant NGAL in monomer and dimer forms adsorbed onto aluminum hydroxide gel (4)
HYB 211-01 is specific for human NGAL. No cross-reaction with NGAL from other species has been detected.
Epitope specificity differs from that of HYB 211-01 and HYB 211-02.
HYB 211-05 reacts strongly with human NGAL. Strong reaction is seen when used as detection antibody in sandwich ELISA in combination with a polyclonal NGAL capture antibody and when tested on recombinant NGAL coated directly onto the well. HYB 211-05 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. In Western blotting of SDS-PAGE gels, HYB 211-05 reacts with NGAL in both reduced and unreduced forms (4).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/200 | 2,3,4,5 |
| Immunoblotting | Yes | 1/50 | 4 |
| Immunohistochemistry | No | 4 |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.5 µg/mL. Users should determine the optimal dilutions for their own purposes.
1. Kjeldsen L, Johnsen AH, Sengeløv H, Borregaard N (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem 268:10425-10432.
2. Nielsen BS, Borregaard N, Bundgaard JR, Timshel S, Sehested M, Kjeldsen L (1996) Induction of NGAL synthesis in epithelial cells of human colorectal neoplasia and inflammatory bowel disease. Gut 38:414-420.
3. Mishra J, Ma Q, Prada A, Mitsnefes M, Zahedi K, Yang J, Barasch J, Devarajan P (2003) Identification of neutrophil gelatinase-associated lipocalin as a novel early urinary biomarker for ischemic renal injury. J Am Soc Nephrol 14:2534-2543
4. Kjeldsen L, Koch C, Arnljots K, Borregaard N (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Meth 198:155-164.
Further examples of use, please consult NGAL reference booklet at www.bioporto.com
Downloads
HYB 211-05.pdf (20.41 kB) Product specification
NGAL human references.pdf (254.83 kB) NGAL human references
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