Anti-Neutrophil gelatinase-associated lipocalin (dog, NGAL)

Mouse monoclonal antibody, biotinylated

Cat.No. ABS 047-14B

Subclass IgG2b/k
Presentation:

Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Dog neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2 or siderocalin) may, by analogy with its homologues in other mammalian species, be released from activated neutrophils in inflammation or infection, from certain epithelial cancers, and more dramatically from renal tubular cells in response to ischemic or nephrotoxic injury.

Immunogen:

Recombinant dog NGAL adsorbed onto aluminum hydroxide gel

Specificity:

ABS 047-17 binds dog NGAL

Epitope specificity:

Not determined

Reactivity:

ABS 047-14 binds free dog NGAL.

ABS 047-14 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 047-17 (as capture antibody) for measuring dog NGAL, giving a detection limit of 5 pg/mL in an unoptimized buffer assay.

Application:
Method Usability References
ELISA Yes
Immunoblotting
Immunohistochemistry

BioPorto - Grusbakken 8, DK-2820 Gentofte, Denmark
Phone: (+45) 4529 0000 - Fax: (+45) 4529 0001 - Email: info@bioporto.com

Created by Wizkids ApShttp://wizkids.dk