Anti Butyrylcholinesterase (human, BtChE)
Mouse monoclonal antibody
Cat.No. HAH 002-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Butyrylcholinesterase (BtChE, EC 3.1.1.8.) is synthetizised in the liver, and is predominantly found in serum, liver and pancreas. Butyrylcholinesterase is a tetrameric glycoprotein (molecular mass of 350 kDa), and consists of four subunits, each with molecular mass of 90 kDa (1).
Butyrylcholinesterase isolated from human plasma
HAH 002-01 has specificity for butyrylcholinesterase from human serum or plasma.
Crossreactivity to BtChE from other species has not been tested.
Not determined
HAH 002-01 reacts with BtChE from normal serum when tested in sandwich ELISA using a polyclonal antibody against BtChE as capture antibody, and in crossed immunoelectrophoresis followed by incubation of the gel with HAH 002-01 (2,3). Serum ChE activity has been measured by enzyme antigen immunoassay (EAIA) in combination with a polyclonal antibody against ChE and HAH 002-01 (4).
In immunoblotting after SDS-PAGE no reaction is seen with neither the reduced nor with the nonreduced form of BtChE. No reaction is seen with acetylcholinesterase from human nervous tissue and erythrocytes.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:8000 | 2 |
| Immunoblotting | No | ||
| Immunohistochemistry | Not determined |
1. Whittaker M: Cholinesterase. Monographs in human genetics 11 (ed. Beckman L.) Karger 1986.
2. Brock A, Mortensen V, Rasmussen Loft AG, Norgaard-Pedersen B (1990) Enzyme immunoassay of human cholinesterase (EC 3.1.1.8). Comparison of immunoreactive substance concentration with catalytic activity concentration in randomly selected serum samples from healthy individuals. J Clin Chem Clin Biochem 28:221-224.
3. Skjodt K, Schou C, Koch C (1984) Assay for the specificity of monoclonal antibodies in crossed immunoelectrophoresis. J Immunol Methods 72:243-249.
4. Hangaard J, Whittaker M, Loft AGR, Norgaard-Pedersen B (1991) Quantification and phenotyping of serum cholinesterase by enzyme antigen immunoassay: methodological aspects and clinical applicability. Scand J Clin Lab Invest 51:349-358.
