Anti-Amyloid ß-peptide (human)
Mouse monoclonal antibody
Cat.No. HYB 310-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Synthetic amyloid ß-peptide (5-16): RHDSGYEVHHQK-NH2 represents part of the larger amyloid ß-peptide that precipitates as amyloid plaques in the brain of patients with Alzheimer's disease (1). The plaque-related peptides are 40-43 amino acid residues in length (2).
Amyloid ß-peptide (5-16) adsorbed onto aluminum hydroxide gel
The binding of HYB 310-08 to amyloid ß-peptide depends on the presence of at least aminoacid residues 10-16.
Epitope specificity differs from that of HYB 310-03 and HYB 310-07
HYB 310-08 was tested by ELISA in wells coated with BSA-coupled synthetic peptides from the amyloid ß-peptide.
HYB 310-08 is suitable for immunoaffinity purification of amyloid ß-peptide (4).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohostochemistry | Not determined |
1. Walsh DM, Klyubin I, Fadeeva JV, Cullen WK, Anwyl R, Wolfe MS, Rowan MJ, Selkoe DJ (2002) Naturally secreted oligomers of amyloid beta protein potently inhibit hippocampal long-term potentiation in vivo. Nature 416:535-539.
2. Abraham CR (2000) Amyloid beta peptide: a century of discoveries. Amyloid 7:7-9.
3. Lewczuk P, Esselmann H, Meyer M, Wollscheid, Neumann M, Otto M, Maler JM, Rüther E, Kornhuber J, Wiltfang J (2003) The amyloid-b (Ab) peptide pattern in cerebrospinal fluid in Alzheimer’s disease: evidence of a novel carboxyterminally elongated Ab peptide. Rapid Commun Mass Spectrom 17:1291-1296.
4. Sen JW, Bergen HR III, Heegaard NH (2003) On-line immunoaffinity-liquid chromatography-mass spectrometry for identification of amyloid disease markers in biological fluids. Anal Chem 75:1196-1202.
