Anti Trypsin (human)
mouse monoclonal antibody
Cat.No. HYB 021-08
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Trypsin is a 24 kDa enzyme of the serine proteinase family. It is produced in the pancreas as an inactive precursor, trypsinogen. The active enzyme is located in the gastrointestinal tract where it degrades proteins to large peptides. Trypsin predominantly cleaves proteins at the carboxyl side of the amino acids lysine and arginine. A common trypsin substrate is chymotrypsinogen which is cleaved to produce active chymotrypsin.
High levels of immunoreactive trypsin in the bloodstream can indicate pancreatic malfunction and this indicator is used to screene for and diagnose Cystic Fibrosis (1).
Trypsin purified from human pancreatic tissue and adsorbed onto aluminum hydroxide gel (2)
HYB 021-08 is specific for human trypsin, trypsinogen and trypsin in complex with alpha-1-antitrypsin. Cross-reacts weakly with bovine trypsin.
Epitope differs from that of HYB 021-03
HYB 021-08 reacts with human trypsin coated directly onto a microtiter plate and is suitable as a detection antibody in sandwich ELISA with a coat of HYB 021-03.
HYB 021-08 recognizes human trypsin in Western blotting under reducing conditions.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/8000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for human trypsin coated at 10 BAEE units/mL. Users should dertermine the optimal dilution for their own purposes
1. Crossley JR, Smith PA, Edgar BW, Gluckman PD, Elliott RB (1981) Neonatal screening for cystic fibrosis, using immunoreactive trypsin assay in dried blood spots. Clin Chim Acta 113:111-121.
2. Feinstein G, Hofstein R, Koifmann J, Sokolovsky M (1974) Human pancreatic proteolytic enzymes and protein inhibitors. Isolation and molecular properties. Eur J Biochem 43:569-581.
