Anti-Alkaline phosphatase

Mouse monoclonal antibody

Cat.No. HYB 252-08

Subclass IgG1/k
Presentation:

Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Alkaline phosphatatase is a hydrolase enzyme responsible for removing phosphate groups (dephosphorylation) from many types of molecules, including nucleotides, proteins, and alkaloids.

Alkaline phosphatase is made in the liver, bone, and the placenta and normally present in high concentrations in growing bone and in bile.

Immunogen:

Alkaline phosphatase from calf intestine adsorbed onto aluminum hydroxide gel

Specificity:

Alkaline phosphatase

Epitope specificity:

Not determined

Reactivity:

When alkaline phosphatase is coupled to streptavidin and immobilized to the solid phase in ELISA, HYB 252-08 binds strongly to the presented alkaline phosphatase.

Application:
Method Usability References
ELISA Yes
Immunoblotting Not determined
Immunohistochemistry Not determined

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