Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Vasoactive intestinal peptide (VIP) is a 28 amino acid peptide belonging to the secretin-glucagon family of peptide hormones. Its sequence is identical in humans, cows, pigs and rats. VIP is widely distributed in the central and peripheral nervous systems, acting as a neurotransmitter or neuromodulator. Peripherally it is involved in vaso- and bronchodilatation, smooth muscle relaxation and stimulation of secretion. Ectopic endocrine tumors secreting VIP (VIPomas) give rise to raised plasma levels of VIP and are associated with watery diarrhea, hypokalemia and achlorhydria syndrome.
VIP shows high sequence homology with peptide histidine-methionine (PHM) in humans or peptide histidine-serine (PHI) in pigs or rats, present in the same precursor, and with the neuropeptide pituitary adenylate cyclase activating polypeptide (PACAP).

Vasoactive intestinal peptide adsorbed onto aluminum hydroxide gel

ABS 023-02 binds to human, bovine, porcine or rat VIP, but does not bind to PHM or PACAP

Not determined

The dilution guideline of 1/1000 applies to VIP coated directly at 2 µg/mL. For glutaraldehyde-linked VIP-ovalbumin (molar ratio 4:1) coated at 2 µg/mL, the dilution is 1/32,000.