Anti PYY(3-36) (peptide tyrosine-tyrosine amide, 3-36)
Mouse monoclonal antibody
Cat.No. ABS 051-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Peptide tyrosine-tyrosine amide (3-36), or PYY(3-36), is a major metabolite of the gut hormone PYY and is produced by the action of dipeptidyl peptidase IV on PYY in the intestinal brush border and in the circulation (1,2). PYY(3-36) is a potent inhibitor of food intake in rats and humans, acting selectively on the Y2 receptor to inhibit orexigenic NPY neurons in the arcuate nucleus, thus disinhibiting anorexigenic POMC neurons (3). The anorexigenic effect of PYY(3-36) is additive to that of the co-secreted gut hormone GLP-1 (4).
Synthetic N-terminal fragment of human PYY(3-36) coupled to carrier
ABS 051-04 binds human PYY(3-36)
Based on inhibition ELISA, ABS 051-04 shows no cross-reaction with PYY(1-36), PYY from mouse, rat, dog or pig, or NPY.
The epitope lies within residues 3-12 of human PYY
ABS 051-04 binds free PYY(3-36) in solution. The ELISA dilution guideline of 1/20,000 applies to PYY(3-36) coated directly at 1 μg/mL. ABS 051-04 as capture antibody forms a sandwich ELISA antibody pair with biotinylated ABS 029-01 as detection antibody for measuring PYY(3-36).
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Yes | |
| Immunohistochemistry | Not determined |
1. Eberlein GA, Eysselein VE, Schaeffer M, Layer P, Grandt D, Goebell H, Niebel W, Davis M, Lee TD, et al (1989) A new molecular form of PYY: structural characterization of human PYY(3-36) and PYY(1-36). Peptides 10:797-803.
2. Grandt D, Dahms P, Schimiczek M, Eysselein VE, Reeve JR Jr, Mentlein R (1993) Proteolytic processing by dipeptidyl aminopeptidase IV generates receptor selectivity for peptide YY (PYY). Med Klin (Munich) 88:143-145.
3. Batterham RL, Cowley MA, Small CJ, Herzog H, Cohen MA, Dakin CL, Wren AM, Brynes AE, Low MJ, Ghatei MA, Cone RD, Bloom SR (2002) Gut hormone PYY(3-36) physiologically inhibits food intake. Nature 2002; 418:650-654.
4. Neary NM, Small CJ, Druce MR, Park AJ, Ellis SM, Semjonous NM, Dakin CL, Filipsson K, Wang F, Kent AS, Frost GS, Ghatei MA, Cone RD, Bloom SR (2005) Peptide YY 3-36 and glucagon peptide 1 7-36 inhibit food intake additively. Endocrinology 146: 5120-5127.
