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Anti-Glucagon-like peptide-1 (GLP-1 (7-36) amide)

Mouse monoclonal antibody

Cat.No. HYB 147-13

Subclass IgG1/k

Presentation:

Preparation: Protein-A purified
Content: Available in 200 無 and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8慢

Antigen:

Glucagon-like peptide 1(7-36)amide (GLP-1 (7-36)amide) is the principal active form of GLP-1, the other being GLP-1 (7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Immunogen:

Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel.

Specificity:

Reacts with all forms of GLP-1, including precursor.

Epitope specificity:

Mid-molecular epitope of GLP-1. Differs from that of HYB 147-06 (C-terminal amide)

Reactivity:

HYB 147-13 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 1.1x10E8 in inhbition ELISA. HYB 147-13 cross-rects <0.6% with coated glucagon. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%.

Application:

Method	Usability	Dilution guideline	References
ELISA	Yes	1/20,000
Immunoblotting	Yes	1/1000
Immunohistochemistry	Not determined

Application Remarks:

The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.1-1 痢/mL. Users should determine the optimal dilutions for their own purposes.

References:

1. Uttenthal LO, Ghiglione M, van Delft J, Garc燰 Hermida O, Fontela T, Koch C (1993) A sensitive enzyme-linked immunosorbent assay for glucagon-like peptide-1. Digestion 54:395-396.
2. Ghiglione M, Uttenthal LO, Koch C (1993) Monoclonal antibodies to glucagon-like peptide-1. Digestion 54:396-397.
3. van Delft J, Uttenthal O, Koch C, Ghiglione M (1999) Immunoblockade of endogenous glucagon-like peptide-1 by monoclonal antibodies in conscious rats: effect on the insulin response to intragastric glucose. Metabolism 48:41-46.