Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Precipitation has occasionally been seen on storage, which has not affected product performance. If necessary, any precipitate can be removed by centrifugation.

Glucagon-like peptide-1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel

Reacts with all forms of GLP-1, including precursor and GLP-1(9-37) /GLP-1(9-36amide) metabolite

Mid-molecular epitope of GLP-1

HYB 147-12 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 4.0x10E8 in inhibition ELISA. HYB 147-12 cross-reacts <0.4% with coated glucagon. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%. Although not tested, HYB 147-12 is likely to detect all known molecular forms of GLP-1 in immunohistochemistry. Biotinylated HYB 147-12 is the preferred detection antibody for measuring C-terminally amidated forms of GLP-1 in combination with HYB 147-06 as capture antibody.
HYB 147-12 can be used as a capture antibody in combination with ABS 046-03B as a detection antibody for measuring non-amidated GLP-1 forms and cross- reacting about 16% with C-terminally amidated GLP-1. Results show detection limits of 44pmol/L which is 10-20 times higher than the basal concentration of GLP-1, so the assays have to be optimized. In Western blotting a dilution guideline of 1/2000 has proved successful.