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Anti-Glucagon-like peptide-1 (GLP-1, Mid-molecule specific)

Mouse monoclonal antibody, biotinylated

Cat.No. HYB 147-08B

Subclass IgG2a/k

Presentation:

Preparation: Biotinylated
Content: 50 μL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Antigen:

Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Immunogen:

Synthetic GLP-1(7-36)amide coupled to carrier and adsorbed onto aluminum hydroxide gel

Specificity:

Reacts with all forms of GLP-1, including precursor and GLP-1(9-37) /GLP-1(9-36amide) metabolite

Epitope specificity:

Mid-molecular epitope of GLP-1

Reactivity:

HYB 147-08 binds to GLP-1 when coated directly onto the microtiter well, and binds GLP-1(7-36)amide in solution giving a Ka of 1.2x10E7 in inhibition ELISA. HYB 147-08 does not cross-react with coated glucagon or hGLP-2. In inhibition ELISA no binding of free glucagon in solution is detected, giving an estimated cross-reactivity of <0.2%. Although not tested, HYB 147-08 is likely to detect all known molecular forms of GLP-1 in immunohistochemistry. In Western blotting a dilution guideline of 1/1000 has proved successful.

Application: