Anti-GLP-1(7-37) and GLP-1(7-36) amide (free N-terminus-specific)
Mouse monoclonal antibody
Cat.No. ABS 033-10
Preparation: Protein-A purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Glucagon-like peptide 1(7-36)amide (GLP-1(7-36)amide) is the principal active form of GLP-1, the other being GLP-1(7-37). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose-dependent insulin secretion. The sequence of of GLP-1 is fully conserved in all mammalian species examined so far.
KLH-conjugated synthetic [Cys18]-GLP-1(7-17) adsorbed onto aluminum hydroxide gel
ABS 033-10 binds the free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide and shows <0.2% cross-reactivity with GLP-1(9-36) amide, glucagon and human GIP. Cross-reacts 0.25% with GLP-1(1-37), approximately 6% with human GLP-2 and approximately 50% with exendin-4.
Free N-terminus of GLP-1(7-37) and GLP-1(7-36) amide.
ABS 033-10 binds free GLP-1(7-37) and free GLP-1(7-36) amide in solution.
ABS 033-10 reacts in ELISA with GLP-1(7-37) coated directly onto the microtiter well.
ABS 033-10 binds free exendin-4 in solution and can function as a capture antibody for exendin-4.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/20,000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
Downloads
ABS 033-10.pdf (19.55 kB)
GLP-1 Flyer.pdf (487.77 kB)
The dilution guideline for ELISA is based on use as detection antibody for GLP-1 (7-37) coated at 1 µg/mL. Users should determine the optimal dilutions for their own purposes.
GLP-1 brochure.pdf (462.46 kB)
GLP-1 brochure
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