Preparation: Protein-A purified

Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide

Storage: 4-8ºC without exposure to light. No precautions necessary during handling.

Ghrelin is an orexigenic (food intake stimulating) 27 or 28 amino-acid gut-brain peptide hormone produced by endocrine cells in the stomach, pancreatic islets and certain hypothalamic neurons1,2. Two forms are produced by alternative mRNA splicing; Gln13 or 14 is missing from the second form. "Active" ghrelin is acylated (octanoylated) at Ser3; this form stimulates growth hormone secretion via GHS receptors and increases appetite. The des-acyl form does not have these effects but may have other actions. Plasma responses of total ghrelin to food reflect those of active ghrelin3.

Synthetic ghrelin (14-28)

ABS 052-121 binds human ghrelin and cross-reacts with rat ghrelin. It is presumed to cross-react with ghrelins of other species with identical 14-28 sequences, such as mouse, dog and cat.

Not determined

ABS 052-121 binds human ghrelin in solution.

ABS 052-121 (as biotinylated detection antibody) forms an antibody pair with ABS 050-16 (as capture antibody) for measuring human ghrelin in sandwich ELISA.