Anti Exendin-4, biotinylated
Mouse monoclonal antibody
Cat.No. ABS 012-35B
Preparation: Biotinylated
Content: Available in 50 µL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.13 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Exendin-4 is a 39-amino-acid peptide found in venom from the Gila monster Helicoderma suspectum. It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion.
Carrier-coupled exendin-4
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells.
There is full reaction with exendin-4 (9-39) when used as coat in ELISA showing an epitope in the 9 - 39 region
ABS 012-35 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.1x10E9 in an inhibition ELISA. The binding between ABS 012-35 and exendin-4 is disrupted by 4.5 M MgCl2.
ABS 012-35 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 033-10 (as capture antibody) for measuring exendin-4, giving a detection limit of as little as 10 pmol/L in an unoptimized buffer assay.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:2000 | |
| Immunoblotting | Not determined | ||
| Immunohistochemistry | Not determined |
1. Eng J, Kleinman WA, Singh L, Singh G, Raufman JP (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom. Further evidence for an exendin receptor on dispersed acini from guinea pig pancreas. J Biol Chem 267:7402-7405.
2. Goke R, Fehmann HC, Linn T, Schmidt H, Krause M, Eng J, Goke B (1993) Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells. J Biol Chem 268:19650-19655.
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