Anti-Exendin-4
Mouse monoclonal antibody
Cat.No. ABS 012-20
Preparation: Protein-A/G purified
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage:4-8ºC without exposure to light. No precautions necessary during handling.
Exendin-4 is a 39 amino acid peptide found in venom from the Gila monster Heloderma suspectum (1). It is a member of the glucagon-secretin family of peptide hormones and neuropeptides. Exendin-4 is a potent agonist of the GLP-1 receptor and hence a potent stimulator of insulin secretion (2).
Synthetic exendin-4 adsorbed onto aluminum hydroxide gel
Specific for exendin-4. No cross-reactivity with GLP-1, GLP-2 (human) or glucagon coated on ELISA wells
The epitope is in the 9-39 region of the peptide, and the antibody cross-reacts strongly with exendin (9-39)amide.
ABS 012-20 binds to exendin-4 when coated on ELISA wells and reacts specifically with exendin-4 in solution giving a Ka of 1.6x10E8 in inhibition ELISA. The binding between ABS 012-20 and exendin-4 is disrupted by 4.5 M MgCl2.
| Method | Usability | References |
| ELISA | Yes | |
| Immunoblotting | Not determined | |
| Immunohistochemistry | Not determined |
1. Eng J, Kleinman WA, Singh L, Singh G, Raufman JP (1992) Isolation and characterization of exendin-4, an exendin-3 analogue, from Heloderma suspectum venom. Further evidence for an exendin receptor on dispersed acini from guinea pig pancreas. J Biol Chem 267:7402-7405.
2. Goke R, Fehmann HC, Linn T, Schmidt H, Krause M, Eng J, Goke B (1993) Exendin-4 is a high potency agonist and truncated exendin-(9-39)-amide an antagonist at the glucagon-like peptide 1-(7-36)-amide receptor of insulin-secreting beta-cells. J Biol Chem 268:19650-19655.
Downloads
ABS 012-20.pdf (31.87 kB) Product specification
GLP-1 and Exendin-4 brochure.pdf (116.14 kB) GLP-1 and Exendin-4 brochure
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