Anti Estradiol-17-beta
mouse monoclonal antibody
Cat.No. HYB 057-02
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide
Storage: In the dark at 4-8ºC
Biologically active oestrogen, estradiol-17beta, is an important sex hormone, but also makes an important contribution to the high concentrations of oestrogens which are present in malignant breast tissues.
Estradiol-17b-6-CMO coupled to BSA
HYB 057-02 reacts with estradiol-17beta. No crossreactivity is seen with estrone, esrriol, estrone sulphate, 16-ketoestradiol, 2-hydroxyestradiol, progesterone, androstenedione, testosterone, or cortisol when measuring on tritium-labeled steroids in radioimmunoassay.
Epitope specific for the hormone, no reactivity to the CMO linker
HYB 057-02 reacts specifically with estradiol-17beta with a measured Ka of 3x10E9 in a radioimmunoassay. HYB 057-02 can be cleaved with papain and reduced to generate Fab fragments with a Ka of 13.4x10E9 (1).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:18000 | |
| Immunoblotting | Not determined | ||
| Immunihistochemistry | Not determined |
1. Pajunen M, Saviranta P, Jauria P, Karp M, Petterson K, Mantsala P, Lovgren T (1997) Cloning, sequencing, expression and characterization of three anti-estradiol-17b Fab fragments. Biochim Biophys Acta 1351:192-202.
2. Lamminmaki U, Villoutreix BO, Jauria P, Saviranta P, Vihinen M, Nilsson L, Teleman O, Lovgren T (1997) Structural analysis of an anti-estradiol antibody. Mol Immunol 34:1215-1226.
3. Saviranta P, Jauria P, Lamminmaki U, Hellman J, Eriksson S, Lovgren T (2000) N-terminal mutations in the anti-estradiol Fab 57-2 modify its hapten binding properties. Protein Sci 9:2547-56.
4. Lamminmaki U, Kankare JA (2001) Crystal structure of a recombinant anti-estradiol Fab fragment in complex with 17b-estradiol. J Biol Chem 276:36687-94.
5. McEwen BS, Davis PG, Parsons B, Pfaff DW (1979) The brain as a target for steroid hormone action. Annu Rev Neurosci 2:65-112.
6. Jorgensen L, Brunner N, Spang-Thomsen M, James MR, Clarke R, Dombernowsky P, Svenstrup B (1997) Steroid metabolism in the hormone dependent MCF-7 human breast carcinoma cell line and its two hormone resistant subpopulations MCF-7/LCC1 and MCF-7/LCC2. J Steroid Biochem Mol Biol 63:275-281.
