Anti Chorionic gonadotrophin (human, hCG)
Mouse monoclonal antibody
Cat.No. HYB 093-04
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL
Concentration: 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
Storage: In the dark at 4-8ºC
Human chorionic gonadotrophin (hCG) is a luteotrophic glycoprotein hormone secreted first by the trophoblastic cells of the fertilized ovum and later by placental tissue (1). The primary function of hCG is its luteotropic effect. hCG consists of non-covalently linked, a and b subunits, 12 kDa and 24 kDa respectively, together constituting holo-hCG (MW 38 kDa) (2).
Human chorionic gonadotrophin (hCG) isolated from urine of women in early pregnancy.
HYB 093-04 is specific for holo-hCG from human serum/plasma or urine. No cross-reaction is seen with human luteinising hormone. Cross-reactivity to chorionic gonadotropin from other species has not been tested.
Epitope specificity differs from HYB 093-05 and HYB 093-09 but overlap, as determined by inhibition ELISA.
HYB 093-04 reacts with hCG when tested in sandwich ELISA using a polyclonal antibody against hCG as capture antibody. In Western blotting after SDS-PAGE HYB 093-04 reacts weakly with SDS-treated intact hCG and only under nonreducing conditions.
No reaction is seen with isolated a-hCG or b-hCG subunits.
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1:4000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
1.Tojo, S (1982) The biology and chemistry of human chorionic gondotropin. In: Pregnancy proteins (Eds) Academic Press 25-38.
2. Schwarz, S (1986) The antigenic surface of human chorionic gonadotropin as mapped by murine monoclonal antibodies. Endocrinology 118:189-97.
3. Norgaard-Pedersen B, Larsen SO, Arends J, Svenstrup B, Tabor A (1990) Maternal serum markers in screening for Down syndrome. Clin Genet 37:35-43.
