BioPorto Diagnostic

AntibodyShop

MBL Oligomer ELISA Kit

Cat.No. KIT 029

Numbers of tests:

96 wells

Assay time:

< 4 hours

Result type:

Quantitative

Regulatory status:

CE IVD

Clinical significance
Mannan-binding lectin (MBL) is a plasma protein that activates the complement system on binding to invading pathogens, and also has more subtle immunomodulatory effects. Genetically determined MBL deficiency has been shown to be associated with increased susceptibility to infections in conditions such as:

Immunosuppression
- During cancer chemotherapy
- After organ transplantation
Immature adaptive immune system
- In early childhood

MBL deficiency is also associated with increased disease severity in:

Autoimmune diseases
- Systemic lupus erythematosus (SLE)
- Rheumatoid arthritis

Activation of the complement system
MBL activates complement through its own pathway, the lectin or MBL pathway, one of the three known complement-activating pathways, the other two being the classical and the alternative pathways.

In about 12% of the average population, the circulating level of functional MBL is too low to activate the complement system. Such MBL-deficient individuals may be more susceptible to infections, especially if the adaptive immune system is immature or suppressed, e.g. by cytotoxic drugs.

Kit features

Highly reproducible and easy to use
- Ready-to-use calibrators and working solutions
- Pre-coated ELISA strips
- All incubations performed at room temperature
- Measures MBL in both serum and plasma
Detection range (At the recommended sample dilutions) 2 – 4000 ng/mL Easy storage
All components are stored at 4°C
CE IVD-marked
Only standard ELISA equipment is required

References:

The AntibodyShop MBL Oligomer ELISA Kit has been used in these references:

Förster-Waldl E, Cokoja L, Föster O, Maurer W (2003) Mannose-binding lectin: comparison of two assays for the quantification of MBL in the serum of pediatric patients. J Immunol Meth 276:143-146.
Seibold F, Konrad A, Flogerzi B, Seibold-Schmid B, Arni S, Jüliger S, Kun JFJ (2004) Genetic Variants of the Mannan-Binding Lectin Are Associated With Immune Reactivity to Mannans in Chrons Disease. Gastroenterology 127:1076-1084.
Megia A, Gallart L, Fernández-Real JM, Vendrell J, Simón I, Gutierrez C, Richart C (2004) Mannose-Binding Lectin Gene Polymorphisms Are Associated with Gestational Diabetes Mellitus. J Clin Endocrinol Metab 89:5081-5087.
Fidler KJ, Wilson P, Davies JC, Turner MW, Peters MJ, Klein NJ (2004) Increased incidence and severity of the systemic inflammatory response syndrome in patients deficient in mannose-binding lectin. Intensive Care Med 30:1438-1445.
Heggelund L, Mollnes TE, Ueland T, Christophersen B, Aukrust P, Froland SS (2003) Mannose-binding lectin in HIV infection: relation to disease progression and highly active antiretroviral therapy. J Acquir Immune Defic Syndr 32:354-361.
Pellis V, De Seta F, Crovella S, Bossi F, Bulla R, Guaschino S, Radillo O, Garred P, Tedesco F (2005) Mannose binding lectin and C3 act as recognition molecules for infectious agents in the vigina. Clin Exp Immunol 139:120-126.
Davies JC, Turner MW, Klein N (2004) Impaired pulmonary status in cystic fibrosis adults with two mutated MBL-2 alleles. Eur Respir J 24:798-804.
Stephens RC, Fidler K, Wilson P, Barclay GR, Mythen MG, Dixon GL, Turner MW, Klein NJ, Peters MJ (2006) Endotoxin immunity and the development of the systemic inflammatory response syndrome in critically ill children. Intensive Care Med. 32:286-294.
Selvaraj P, Jawahar MS, Rajeswari DN, Alagarasu K, Vidyarani M, Narayanan PR (2006) Role of mannose binding lectin gene variants on its protein levels and macrophage phagocytosis with live Mycobacterium tuberculosis in pulmonary tuberculosis. FEMS Immunol Med Microbiol. 46:433-7.
Garcia-Laorden MI, Pena MJ, Caminero JA, Garcia-Saavedra A, Campos-Herrero MI, Caballero A, Rodriguez-Gallego C (2006) Influence of mannose-binding lectin on HIV infection and tuberculosis in a Western-European population. Mol Immunol. 2006 Feb 22; [Epub ahead of print]
Inanc N, Mumcu G, Birtas E, Elbir Y, Yavuz S, Ergun T, Fresko I, Direskeneli H (2005) Serum Mannose-Binding Lectin Levels Are Decreased in Behcet’s Disease and Associated with Desease Severity. J Rheumatol 32:287-291.
Siassi M, Riese J, Steffensen R, Meisner M, Thiel S, Hohenberger W, Schmidt J (2005) Mannan-binding lectin and procalcitonin measurement for prediction of postoperative infection. Critical Care 9: R483-R489.