Anti-Mannan-binding lectin (MBL, chicken)
Mouse monoclonal antibody
Cat.No. HYB 182-01
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide
Storage: 4-8ºC without exposure to light. No precautions necessary during handling.
Mannan-binding lectin (MBL) belongs to the C-type family of collectins. It functions as an opsonin which activates complement on binding to microbial polysaccharides. Studies on chicken MBL are described in refs 1-4.
MBL purified from chicken plasma and adsorbed onto aluminum hydroxide
HYB 182-01 is specific for chicken MBL
Not determined
In ELISA HYB 182-01 reacts strongly with chicken MBL. HYB 182-01 can be used as coating antibody (5 µg/mL) with biotinylated HYB 182-01 as detection antibody (1 µg/mL) in sandwich ELISA (1-3). In Western blotting a dilution guideline of 1/1000 has proved successful (4).
| Method | Usability | References |
| ELISA | Yes | 3 |
| Immunoblotting | Yes | 4 |
| Immunohistochemistry | Yes | 4 |
1.Laursen SB, Hedemand JE, Nielsen OL, Thiel S, Koch C, Jensenius JC (1998) Serum levels, ontogeny and heritability of chicken mannan-binding lectin (MBL). Immunology 94:587-593.
2. Nielsen OL, Jensenius JC, Jorgensen PH, Laursen SB (1999) Serum levels of chicken mannan-binding lectin (MBL) during virus infections; indication that chicken MBL is an acute phase reactant. Vet Immunol Immunopathol 70:309-316.
3. Juul-Madsen HR, Munch M, Handberg KJ, Sorensen P, Johnson AA, Norup LR, Jorgensen PH (2003) Serum levels of mannan-binding lectin in chickens prior to and during experimental infection with avian infectious bronchitis virus. Poult Sci 82:235-241.
4. Nielsen OL, Jorgensen PH, Hedemand J, Jensenius JC, Koch C, Laursen SB (1998) Immunohistochemical investigation of the tissue distribution of mannan-binding lectin in non-infected and virus-infected chickens. Immunology 94:122-128.
Downloads
HYB 182-01.pdf (30.14 kB) Product specification
ELISA Kits and Monoclonal antibodies for the Complement system - flyer.pdf (637.76 kB) ELISA Kits and Monoclonal antibodies for the Complement system
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