Anti-Mannan-binding lectin (human, MBL)
mouse monoclonal antibody
Cat.No. HYB 131-11
Preparation: Protein-A/G purified
Content: Available in 200 µL and 1 mL volumes, 1 mg/mL
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15mM sodium azide Storage: In the dark at 4-8ºC
Mannan-binding lectin (MBL), also called mannose-binding lectin or protein, belongs to the C-type family of collectins, showing calcium-dependent binding to certain sugars (1). It consits of oligomers of triple-chain subunits and its binding and complement activating activities depend on its normal oligomerization. On binding to mannan-like microbial surface carbohydrates, MBL activates the complement system by means of its own lectin pathway, depending on the MBL-associated serine proteases (MASPs). Because of the presence of different structural and promoter alleles in the population, 12% or more of the population have low concentrations (<50ng/mL) of normally oligomerized, functional MBL in plasma or serum (2)
MBL purified from human donor plasma and adsorbed onto aluminum hydroxide.
HYB 131-11 is specific for MBL from human serum or plasma
The epitope is thought to be on the carbohydrate recognition domain and differs from that of HYB 131-01 and HYB 131-10
HYB 131-11 reacts strongly with MBL. Strong reaction is seen in ELISA with MBL coated directly onto the microtiter well. In Western blotting, HYB 131-11 reacts with human MBL in both its oligomerized state and as a single protein chain of 26 kDa. HYB 131-11 can be used to measure MBL in plasma or serum and is specific for oligomerized MBL when used in combination with itself, but not with other MBL antibodies, when it also reacts with poorly oligomerized forms (3).
| Method | Usability | Dilution guideline | References |
| ELISA | Yes | 1/10,000 | |
| Immunoblotting | Yes | ||
| Immunohistochemistry | Not determined |
The dilution guideline for ELISA is based on use as detection antibody for antigen coated at 0.3 µg/mL together with human albumin 8.5 µg/mL. Suggested dilution when used as capture antibody in a sandwich ELISA is 0.3 µg/mL.
1. Kawasaki N, Kawasaki T, Yamashina I (1983) Isolation and characterization of a mannan-binding protein from human serum. J Biochem (Tokyo) 94:937-947.
2. Steffensen R, Thiel S, Varming K, Jersild C, Jensenius JC (2000) Detection of structural gene mutations and promoter polymorphisms in the mannan-binding lectin (MBL) gene by polymerase chain reaction with sequence-specific primers. J Immunol Methods 241:33-42.
3. Garred P, Larsen F, Madsen HO, Koch C (2003) Mannose-binding lectin deficiency - revisited. Mol Immunol 40:73-84
Downloads
HYB 131-11.pdf (21.63 kB) Product specification
Complement flyer.pdf (422.22 kB) Complement flyer
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April 23 2008
